Freezing and thawing techniques can gradually regenerate cells in the right leg, or cause cell death in a CO2 incubator. Refrigerated cells shall be carried out at the rate of one degree per minute with corresponding slow speed refrigeration equipment. This creates a symmetrical freeze between cells and limits the production of frost. Reliable refrigeration unit will keep the cells in a constant state, and it is not easy to cause serious freeze-thaw.
Conversely, unfreezing should be carried out rapidly in a 37 degree water bath and then floated again in a thorough growth and development medium. Establish a standardized protocol to maintain the cell condition. To ensure that they are followed accurately, there is no need to deviate from the on-time procedure flow, resulting in a lot of time in the water bath, constant temperature and humidity mold incubator or other harmful delay time.
It is strongly required that the basic habit of environmental protection monitoring should be used to detect the cleanliness of the laboratory. An agar tablet computer open to the outside is reserved in the cell culture cover of the thermostatic mold incubator. The cyclone is excellent, and then the tablet computer is incubated. It will be obvious if the cover is properly cleaned. The incubator can be used to carry out the same test, and the open culture solution bottle can be stored for a period of time. Ensure that the application has a good layer Flow high-quality ultra clean workbench is the main feature of all cell culture laboratories to maintain the physical and mental health of cell culture without being harmed by the external environment.
Carrying out incubator inspection on CO2 and temperature changes can be the main level to maintain cell happiness. If their environmental factors are unstable, they will not. Therefore, selecting a high-quality incubator that can maintain a constant CO2 level and temperature range can save time and uncertainty.
It is necessary to maintain the time, care and adaptability of cell culture, so as to create a suitable natural environment for the excellent growth and development of cells. Once grasped, cell culture for physical and mental health may be important for successful experiments.
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