Contaminated molds can cause cell weakening and death, or change the expression of harmful test results and transfection efficiency. Chlamydia is a test for cell modeling laboratory, because it is not easy to detect. Mycoplasma detection and measurement based on PCR can be carried out, or nuclear staining can be used to observe the spotted staining on the cell membrane of Chlamydia. All molds should be tested for Mycoplasma before application, especially if received from another laboratory.
In order to better resist pollution, pen / javelin is a common addition to the news media. However, we should pay special attention not to use antibiotics, because work pressure will enhance cell selection and personal behavior. Pen /strep can also mask the pollution problem and make it spread. In turn, ensure that aseptic techniques are used first to avoid all contamination.
Aseptic technology may be a key aspect of BOD biochemical incubator in all cell culture schemes. Apply 70% ethyl acetate spray paint cover successively, spray glue gloves before entering the cover, and spray substance bottles before opening. Various aseptic techniques can keep cells happy and pollution-free without the application of working pressure pen / drive chain.
Know your cells
In order to better maintain a pleasant culture and art, please use the full temperature oscillation incubator from the beginning to the end or gradually from the highly recommended news media. Some cell types reproduce more strongly in special media suitable for them. However, no matter what kind of substance is used, some test standards will cause cell damage. Sparsely injected cells are unhealthy, because the decrease in the total number of cell growth factors and other growth hormones is released by a small number of cells. In order to get rid of this problem better, if the cells of the watchplate must be scarce, the carbon dioxide cell incubator should use 20% standard medium and 80% new medium to fuse when subculturing the cells, so as to give the original buzz of growth hormone and make the cells feel comfortable in the new field.
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